Metagenomic Annotation WorkshopΒΆ

In this part of the metagenomics workshop we will:

  • Translate nucleotides into amino acid seuquences using EMBOSS
  • Annotate metagenomic reads with Pfam domains
  • Discuss and perform normalization of metagenomic counts
  • Take a look at different gene abundance analysis

The workshop has the following exercises:

  1. Translation Exercise
  2. HMMER Exercise
  3. Normalization Exercise
  4. Differential Exercise
  5. BonusExercise: Metaxa2

At least a basic knowledge of how to work with the command line is required otherwise it will be very difficult to follow some of the examples. Have fun!